FEBS Advanced Lecture Course

Matrix Pathobiology, Signaling and Molecular Targets

May 21-26, 2007, Patras, Greece

FEBS Advanced Lecture Course

Matrix Pathobiology, Signaling and Molecular Targets

Conference and Cultural Center, University of Patras

21-26 May, 2007





Monday, 21 May

14:30               • Transportation from Athens Airport to Patras


17:00-18:30     • Registration at the Conference & Cultural Center / Coffee


Chairperson: C. Antonopoulos


18:30-19:00     • Welcome Addresses

K. Koubias, Rector of the University of Patras

K. Kuchler, Chairman of the FEBS Advanced Courses Committee
C.Y. Frangides, Vice Prefect of Achaia

J. Matsoukas, Head of the Department of Chemistry
N. Karamanos, Chairman of the Organizing Committee, President of HSBMB

Honorary Medal Awards to Prof. D. Heinegård and J.–P. Pujol
granted by the Rector of the University of Patras


Invited Opening Lectures (L1 - L2)

19:00-19:40     D. Heinegård (Lund University, Sweden)

Matrix Structure and Assembly

19:40-20:20     K. Kadler (University of Manchester, UK)

Contributions of protein self-assembly and cell-mediated protein secretion to tissue assembly


20:30               • Welcome Reception at the Conference & Cultural Center

21:30               • Transportation to Hotels



Tuesday, 22 May

08:30               • Transportation from Hotels to Conference & Cultural Center


Chairperson/discussion leader: J. Turnbull


• Invited General Lectures / Tutorials (L3 – L5)

09:00-09:40     F. Lamari (University of Patras, Greece)

Glycosaminoglycan Structure Analysis and Biological Roles

09:40-10:20     A. Passi (University of Insubria, Italy)

Structure and Function of Hyaluronan

10:20-11:00     A. Theocharis (University of Patras, Greece)

Structure and Functions of Proteoglycans


11:00-11:30     Coffee break


• Selected talks (ST1 – ST4)

11:30-11:40     Y. Ahmed (University of Liverpool, UK)

Investigating the specificity of heparin/ heparan sulfate activity and protein interactions using saccharide libraries

11:40-11:50     M. Skidmore (University of Liverpool, UK)

Disruption of Plasmodium falciparum mediated rosettes, a symptom of severe malaria, using  chemically modified heparin saccharides.

11:50-12:00    C. Malavaki (University of Patras, Greece)

Charge density distribution of chondroitin sulphate and identification of hyaluronan in chondroitin sulphate preparations by capillary electrophoresis

12:00-12:30     M. Zaman (University of Texas at Austin, USA)

Multi-scale modeling of cell matrix interactions

12:30-13:00     A. Tunnicliffe (Millipore Bioscience Division, Germany)

Multiplex Technology and Applications


13:00-14:30     • Lunch


14:30-16:00     • Poster Session (I) / Coffee


Chairperson/discussion leader: A. Hjerpe


• Invited General Lectures / Tutorials (L6 – L7)

16:00-16:40     G. Panayotou (Fleming Institute, Greece)

Signal Transduction by Growth Factor Receptors

16:40-17:20     D. Thanos (Academy of Athens, Greece)

Mechanisms of Gene expression in higher Eukaryotes


17:20-18:00     Coffee break


18:00-18:40     E. Papadimitriou (University of Patras, Greece)

Angiogenesis in Health and Diseases


• Selected talks (ST5 – ST6)

18:40-18:50     L. Boisen (University of Aarhus, Denmark)

Ageing and angiogenesis

18:50-19:00     M. Raoudi (University of Caen, France)

Chondroitin sulfate stimulates synthesis of high molecular weight hyaluronan in synoviocytes and articular chondrocytes – possible involvement of ERK signaling


19:00               • Dinner

20:00               • Tribute to Simon and Garfunkel – Live by TumbleWeed

22:00               • Transportation to Hotels



Wednesday, 23 May

08:30               • Transportation from Hotels to Conference and Cultural Center


Symposium 1: Cell Adhesion and Matrix-mediated Signaling

Chairperson/discussion leader: M. Humphries


• Invited Lectures (L9 - L10)

9:00-9:40         M. Humphries (University of Manchester, UK)

Syndecan-4 Dependent Rac 1 Regulation Determines Directional Migration in Response to the Extracellular Matrix

9:40-10:20       J. Turnbull (University of Liverpool, UK)

Heparan Sulfate Specificity for Regulation of Signaling and Interaction with Growth Factors

10:20-11:00     K. Sugahara (Hokkaido University, Japan)

Glycobiology of Proteoglycan Signaling in Brain Development


11:00-11:30     • Coffee break


11:30-12:10     R. Fässler (Max Plank Institute, Germany)

The RGD motif in fibronectin is essential for development but dispensable for fibril assembly

12:10-12:50     C. Zervas (Academy of Athens, Greece)

Functions of the Integrin-Actin Linker Complex in Drosophila Development


• Selected talks (ST7)

12:50-13:00     K. Vuoriluoto (University of Turku Centre for Biotechnology, Finland)

Syndecans function as integrin a2b1 co-receptors in adhesion, actin stress fiber assembly and matrix contraction


13:00-23:00     • Lunch / Guided excursion tour to Delfi and dinner at Nafpaktos port (optional)



Thursday, 24 May

08:30               • Transportation from Hotels to Conference and Cultural Center


Symposium 2: Functional ECM Molecules that Influences Health and Diseases

Chairperson/discussion leader: V. Hascall


• Invited Lectures (L14 – L17)

9:00-9:40         V. Hascall (Lerner Research Institute, USA)

Hyaluronan Matrices and Inflammation 

9:40-10:20       P. Heldin (Ludwig Institute, Sweden)

Growth Factor Regulation of Hyaluronan Synthesis: Importance of Hyaluronan-CD44 Interaction in Cell Regulation

10:20-11:00     I. Vlodavsky (Cancer, Vascular & Biology Research Center, Israel)

Regulation and Involvement of Heparanase in Cancer Metastasis and Angiogenesis


11:00-11:30     • Coffee break


11:30-12:10     A. Passi (University of Insubria, Italy)

Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Migration of Smooth Muscle Cells


• Selected talks (ST8 – ST12)

12:10-12:20     A. Genasetti (University of Insubria,  Italy)

Hyaluronan synthesis and human endothelial cell behaviour

12:20-12:30     C. Richter (Institute for Biological Interfaces, Karlsruhe, Germany)

Modification of hyaluronic acid to control cell adhesion

12:30-12:40     T. Jokela (University of Kuopio, Finland)

Monocyte adherence to keratinocyte hyaluronan

12:40-12:50     S. Skandalis (Ludwig Institute for Cancer Research, Uppsala University, Sweden )

Role of hyaluronan-CD44 interactions for breast cancer cell transendothelial migration and PDGF ß-receptor activation

12:50-13:00     N. Ziouti (University of Patras, Greece)

Vitreous and Tears Enzymatic Activities in Experimental Endophthalmitis


13:00-14:30     • Lunch


14:30-16:00     • Poster Session (II) / Coffee


Symposium 3: Organization of ECM

Chairperson/discussion leader: D. Heinegård


• Invited Lectures (L18 - L20)

16:00-16:40     D. Heinegård (Lund University, Sweden)

Extracellular Matrix Molecules in Tissue Breakdown

16:40-17:20     K. Kadler (University of Manchester, UK)

Trafficking, cellular assembly and structure of collagen fibrils

17:20-18:00     C. Kielty (University of Manchester, UK)

Fibrillin Microfibris Assembly and Function


18:00-18:30     • Coffee break


• Selected talks (ST13 – ST17)

18:30-18:40     V. Tillgren (University of Lund, Sweden)

The tyrosine sulphate rich N-terminal domains of the LRR-proteins fibromodulin and osteoadherin display heparin activity and bind proteins with the typical motifs

18:40-18:50     J. Yu (University of Oxford, UK)

Microfibril network organisation in young and aged human intervertebral disc

18:50-19:00     C. Gruppi (University of Pavia, Italy)

Collagens-proteoglycans interactions: type I collagen has multiple binding sites for biglycan

19:00-19:10     A. Kantola (University of Helsinki, Finland)

Fibronectin regulates the assembly of Latent TGF-b Binding Protein 4 (LTBP-4) into the extracellular matrix

19:10-19:20    A. Salo (University of Oulu, Finland)

Lysyl hydroxylase 3, a multifunctional enzyme with lysyl hydroxylase and glycosyltransferase activities, is located also in the extracellular space


19:30-22:30    • Guided excursion to winery ACHAIA CLAUSS /  Dinner



Friday, 25 May

08:30               • Transportation from Hotels to Conference and Cultural Center           


Symposium 4: ECM Pathobiology and Molecular Targets

Chairperson/discussion leader: I. Vlodavsky


• Invited Lectures (L21-L23)

9:00-9:40         R. Perris (University of Parma, Italy)

NG2 Proteoglycan Controls Tumour Cell-Host Microenvironment Interactions Conducive to Metastasis and Serves as Immunotherapeutic Target

9:40-10:20       E. Papadimitriou (University of Patras, Greece)

Heparin-Affin Binding Peptide/Pleitrophin as a New Target for Therapy of Tumors and/or other Angiogenesis-Based Diseases

10:20-11:00     D. Kletsas (NCSR “Demokritos”, Greece)

Transforming Growth Factor-β in Tissue Homeostasis


11:00-11:30     • Coffee break


• Selected talks (ST18 – ST24)

11:30-11:45     H. Pratsinis (Institute of Biology, NCSR “Demokritos”, Greece)

Proliferative Response of Intervertebral Disc Cells to Growth Factors: Interplay with the extracellular milieu

11:45-12:00     S. Misra  (Department of Cell Biology and Anatomy, MUSC, Charleston, SC)
Regulation of COX-2-mediated oncogenesis in colon cancer by a positive feedback  regulatory  loop involving hyaluronan, ERBB2, AKT and

12:00-12:15     K. Dobra (Karolinska Institutet, Sweden)

Targeting Thioredoxin Reductase – a possible new treatment for malignant mesothelioma

12:15-12:30     S. Ghatak (Department of Cell Biology and Anatomy, MUSC, Charleston, SC)

Tissue specific shrna delivery: a novel approach for gene therapy in colon and prostate cancer

12:30-12:40     G. Koliakos (Aristotle University of Thessaloniki, Greece)

ΝΗΕ1: a molecular target for signalling and cell-matrix interactions

12:40-12:50     A. Roussidis (University of Patras, Greece)

Expression and intracellular signaling of PDGF-R in breast cancer cells: evaluation as potent molecular target and effects of the molecular inhibitor STI571

12:50-13:00     O. Kousidou (University of Patras, Greece)

Estradiol as a key factor affecting the synthesis of matrix proteoglycans implicated in breast cancer


13:00-14:30     • Lunch


Symposium 5: ECM Alterations in Health and Disease

Chairperson/discussion leader: K. Sugahara


• Invited Lectures (L24-L26)

14:30-15:10     A. Hjerpe (Karolinska Institute, Sweden)

Malignant Mesothelioma – a Connective Tissue Tumour with Proteoglycan-depending Differentiation Patterns

15:10-15:50     I. Pucci-Minafra (University of Palermo, Italy)

Proteomic modulation in breast cancer cells induced by microenvironment components

15:50-16:30     N. Karamanos (University of Patras, Greece)

Differential Expression and Structural Modifications of Proteoglycans and Metalloproteinases in Cancer – Molecular Targets


16:30-17:00     • Coffee break


• Selected talks (ST25-ST33)

17:00-17:10     A. Zafiropoulos (University of Crete, Greece)

MG63 osteosarcoma cells evade the decorin-induced growth arrest by the protracted expression and activation of EGFR

17:10-17:20     E. Hadler-Olsen  (University of Tromsø, 9037 Tromsø, Norway)

Orthotopic and heterotopic growth of cancer cells, - a mouse model to study the influence of extracellular matrix on the expression of MMPs

17:20-17:30     R. Elenjord (University of Tromsø, Norway)

Bisphosponates down-regulate the expression of matrix metalloproteinases in osteosarcoma cell lines

17:30-17:40     I. Kanakis (University of Patras, Greece)

Correlation between inhibition of osteoclastic bone resorption by zoledronic acid and reduction of serum levels of hyaluronan in breast cancer patients with skeletal metastasis

17:40-17:50     D. Vynios (University of Patras, Greece)

Biochemical and molecular studies of extracellular macromolecular components in squamous cell laryngeal carcinoma

17:50-18:00     N. Malla (University of Tromsø, Norway)

Covalent binding of proMMP-9 to proteoglycan core protein affects the interaction with collagen I and gelatine

18:00-18:10     N. Obermajer (University of Ljubljana, Slovenia)

Immunosupressive activity of cysteine protease cathepsin x via activation of beta-2 integrins

18:10-18:20     S. Tamkovich (Institute of Chemical Biology and Fundamental Medicine, Russia)

 Influence of protease activity on DNA concentration in human blood

18:20-18:30     M. Lapierre (INSERM, Paris, 75010, France)

Opposing role of the proprotein convertases Furin and PACE4 on breast cancer cells malignant phenotypes: Role of TIMP-1


19:30              • Dinner
22:00              • Transportation to Hotels



Saturday, 26 May

08:30               • Transportation from Hotels to Conference and Cultural Center  

Symposium 6: Ageing / Cancer and Proteases

Chairperson/discussion leader: H. Nagase


• Invited Lectures (L27-L29)

09:00-09:40     J. P. Pujol (University of Caen, France)

Molecular Mechanisms of Cartilage Destruction and Repair

09:40-10:20     H. Nagase   (Imperial College London, UK)

Elucidating the Functions of Non-Catalytic Domains of Metalloproteinases that Degrade Extracellular Matrix

10:20-11:00     S. Menashi (CNRS , Universite Paris 12, Creteil, France)

Role of the Matrix Metalloproteinases Inducer EMMPRIN in Wound Healing


• Selected talks (ST34)

11:00-11:10     N. H. Lim (Imperial College London,UK)

Investigation of the effect of ADAMTS-selective TIMP mutants on the aggrecanolytic and collagenolytic activity in human osteoarthritic cartilage


11:10-11:20     • D. Heinegård and J.P. Pujol will deliver the final keynote address to conclude FEBS-MPST2007 Course


11:20-12:00     • Farewell Party


12:00               • Transportation from Patras to Athens Airport


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