FEBS Advanced Lecture Course
Matrix Pathobiology, Signaling and Molecular Targets
Conference and Cultural Center, University of Patras
21-26 May, 2007
Programme
Monday, 21 May
14:30 • Transportation from Athens Airport to Patras
17:00-18:30 • Registration at the Conference & Cultural Center / Coffee
Chairperson: C. Antonopoulos
18:30-19:00 • Welcome Addresses
K. Koubias, Rector of the University of Patras
K. Kuchler, Chairman of the FEBS Advanced Courses Committee
C.Y. Frangides, Vice Prefect of Achaia
J. Matsoukas, Head of the Department of Chemistry
N. Karamanos, Chairman of the Organizing Committee, President of HSBMB
Honorary Medal Awards to Prof. D. Heinegård and J.–P. Pujol
granted by the Rector of the University of Patras
• Invited Opening Lectures (L1 - L2)
19:00-19:40 D. Heinegård (Lund University, Sweden)
Matrix Structure and Assembly
19:40-20:20 K. Kadler (University of Manchester, UK)
Contributions of protein self-assembly and cell-mediated protein secretion to tissue assembly
20:30 • Welcome Reception at the Conference & Cultural Center
21:30 • Transportation to Hotels
Tuesday, 22 May
08:30 • Transportation from Hotels to Conference & Cultural Center
Chairperson/discussion leader: J. Turnbull
• Invited General Lectures / Tutorials (L3 – L5)
09:00-09:40 F. Lamari (University of Patras, Greece)
Glycosaminoglycan Structure Analysis and Biological Roles
09:40-10:20 A. Passi (University of Insubria, Italy)
Structure and Function of Hyaluronan
10:20-11:00 A. Theocharis (University of Patras, Greece)
Structure and Functions of Proteoglycans
11:00-11:30 • Coffee break
• Selected talks (ST1 – ST4)
11:30-11:40 Y. Ahmed (University of Liverpool, UK)
Investigating the specificity of heparin/ heparan sulfate activity and protein interactions using saccharide libraries
11:40-11:50 M. Skidmore (University of Liverpool, UK)
Disruption of Plasmodium falciparum mediated rosettes, a symptom of severe malaria, using chemically modified heparin saccharides.
11:50-12:00 C. Malavaki (University of Patras, Greece)
Charge density distribution of chondroitin sulphate and identification of hyaluronan in chondroitin sulphate preparations by capillary electrophoresis
12:00-12:30 M. Zaman (University of Texas at Austin, USA)
Multi-scale modeling of cell matrix interactions
12:30-13:00 A. Tunnicliffe (Millipore Bioscience Division, Germany)
Multiplex Technology and Applications
13:00-14:30 • Lunch
14:30-16:00 • Poster Session (I) / Coffee
Chairperson/discussion leader: A. Hjerpe
• Invited General Lectures / Tutorials (L6 – L7)
16:00-16:40 G. Panayotou (Fleming Institute, Greece)
Signal Transduction by Growth Factor Receptors
16:40-17:20 D. Thanos (Academy of Athens, Greece)
Mechanisms of Gene expression in higher Eukaryotes
17:20-18:00 • Coffee break
18:00-18:40 E. Papadimitriou (University of Patras, Greece)
Angiogenesis in Health and Diseases
• Selected talks (ST5 – ST6)
18:40-18:50 L. Boisen (University of Aarhus, Denmark)
Ageing and angiogenesis
18:50-19:00 M. Raoudi (University of Caen, France)
Chondroitin sulfate stimulates synthesis of high molecular weight hyaluronan in synoviocytes and articular chondrocytes – possible involvement of ERK signaling
19:00 • Dinner
20:00 • Tribute to Simon and Garfunkel – Live by TumbleWeed
22:00 • Transportation to Hotels
Wednesday, 23 May
08:30 • Transportation from Hotels to Conference and Cultural Center
Symposium 1: Cell Adhesion and Matrix-mediated Signaling
Chairperson/discussion leader: M. Humphries
• Invited Lectures (L9 - L10)
9:00-9:40 M. Humphries (University of Manchester, UK)
Syndecan-4 Dependent Rac 1 Regulation Determines Directional Migration in Response to the Extracellular Matrix
9:40-10:20 J. Turnbull (University of Liverpool, UK)
Heparan Sulfate Specificity for Regulation of Signaling and Interaction with Growth Factors
10:20-11:00 K. Sugahara (Hokkaido University, Japan)
Glycobiology of Proteoglycan Signaling in Brain Development
11:00-11:30 • Coffee break
11:30-12:10 R. Fässler (Max Plank Institute, Germany)
The RGD motif in fibronectin is essential for development but dispensable for fibril assembly
12:10-12:50 C. Zervas (Academy of Athens, Greece)
Functions of the Integrin-Actin Linker Complex in Drosophila Development
• Selected talks (ST7)
12:50-13:00 K. Vuoriluoto (University of Turku Centre for Biotechnology, Finland)
Syndecans function as integrin a2b1 co-receptors in adhesion, actin stress fiber assembly and matrix contraction
13:00-23:00 • Lunch / Guided excursion tour to Delfi and dinner at Nafpaktos port (optional)
Thursday, 24 May
08:30 • Transportation from Hotels to Conference and Cultural Center
Symposium 2: Functional ECM Molecules that Influences Health and Diseases
Chairperson/discussion leader: V. Hascall
• Invited Lectures (L14 – L17)
9:00-9:40 V. Hascall (Lerner Research Institute, USA)
Hyaluronan Matrices and Inflammation
9:40-10:20 P. Heldin (Ludwig Institute, Sweden)
Growth Factor Regulation of Hyaluronan Synthesis: Importance of Hyaluronan-CD44 Interaction in Cell Regulation
10:20-11:00 I. Vlodavsky (Cancer, Vascular & Biology Research Center, Israel)
Regulation and Involvement of Heparanase in Cancer Metastasis and Angiogenesis
11:00-11:30 • Coffee break
11:30-12:10 A. Passi (University of Insubria, Italy)
Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Migration of Smooth Muscle Cells
• Selected talks (ST8 – ST12)
12:10-12:20 A. Genasetti (University of Insubria, Italy)
Hyaluronan synthesis and human endothelial cell behaviour
12:20-12:30 C. Richter (Institute for Biological Interfaces, Karlsruhe, Germany)
Modification of hyaluronic acid to control cell adhesion
12:30-12:40 T. Jokela (University of Kuopio, Finland)
Monocyte adherence to keratinocyte hyaluronan
12:40-12:50 S. Skandalis (Ludwig Institute for Cancer Research, Uppsala University, Sweden )
Role of hyaluronan-CD44 interactions for breast cancer cell transendothelial migration and PDGF ß-receptor activation
12:50-13:00 N. Ziouti (University of Patras, Greece)
Vitreous and Tears Enzymatic Activities in Experimental Endophthalmitis
13:00-14:30 • Lunch
14:30-16:00 • Poster Session (II) / Coffee
Symposium 3: Organization of ECM
Chairperson/discussion leader: D. Heinegård
• Invited Lectures (L18 - L20)
16:00-16:40 D. Heinegård (Lund University, Sweden)
Extracellular Matrix Molecules in Tissue Breakdown
16:40-17:20 K. Kadler (University of Manchester, UK)
Trafficking, cellular assembly and structure of collagen fibrils
17:20-18:00 C. Kielty (University of Manchester, UK)
Fibrillin Microfibris Assembly and Function
18:00-18:30 • Coffee break
• Selected talks (ST13 – ST17)
18:30-18:40 V. Tillgren (University of Lund, Sweden)
The tyrosine sulphate rich N-terminal domains of the LRR-proteins fibromodulin and osteoadherin display heparin activity and bind proteins with the typical motifs
18:40-18:50 J. Yu (University of Oxford, UK)
Microfibril network organisation in young and aged human intervertebral disc
18:50-19:00 C. Gruppi (University of Pavia, Italy)
Collagens-proteoglycans interactions: type I collagen has multiple binding sites for biglycan
19:00-19:10 A. Kantola (University of Helsinki, Finland)
Fibronectin regulates the assembly of Latent TGF-b Binding Protein 4 (LTBP-4) into the extracellular matrix
19:10-19:20 A. Salo (University of Oulu, Finland)
Lysyl hydroxylase 3, a multifunctional enzyme with lysyl hydroxylase and glycosyltransferase activities, is located also in the extracellular space
19:30-22:30 • Guided excursion to winery ACHAIA CLAUSS / Dinner
Friday, 25 May
08:30 • Transportation from Hotels to Conference and Cultural Center
Symposium 4: ECM Pathobiology and Molecular Targets
Chairperson/discussion leader: I. Vlodavsky
• Invited Lectures (L21-L23)
9:00-9:40 R. Perris (University of Parma, Italy)
NG2 Proteoglycan Controls Tumour Cell-Host Microenvironment Interactions Conducive to Metastasis and Serves as Immunotherapeutic Target
9:40-10:20 E. Papadimitriou (University of Patras, Greece)
Heparin-Affin Binding Peptide/Pleitrophin as a New Target for Therapy of Tumors and/or other Angiogenesis-Based Diseases
10:20-11:00 D. Kletsas (NCSR “Demokritos”, Greece)
Transforming Growth Factor-β in Tissue Homeostasis
11:00-11:30 • Coffee break
• Selected talks (ST18 – ST24)
11:30-11:45 H. Pratsinis (Institute of Biology, NCSR “Demokritos”, Greece)
Proliferative Response of Intervertebral Disc Cells to Growth Factors: Interplay with the extracellular milieu
11:45-12:00 S. Misra (Department of Cell Biology and Anatomy, MUSC, Charleston, SC)
Regulation of COX-2-mediated oncogenesis in colon cancer by a positive feedback regulatory loop involving hyaluronan, ERBB2, AKT and b-catenin
12:00-12:15 K. Dobra (Karolinska Institutet, Sweden)
Targeting Thioredoxin Reductase – a possible new treatment for malignant mesothelioma
12:15-12:30 S. Ghatak (Department of Cell Biology and Anatomy, MUSC, Charleston, SC)
Tissue specific shrna delivery: a novel approach for gene therapy in colon and prostate cancer
12:30-12:40 G. Koliakos (Aristotle University of Thessaloniki, Greece)
ΝΗΕ1: a molecular target for signalling and cell-matrix interactions
12:40-12:50 A. Roussidis (University of Patras, Greece)
Expression and intracellular signaling of PDGF-R in breast cancer cells: evaluation as potent molecular target and effects of the molecular inhibitor STI571
12:50-13:00 O. Kousidou (University of Patras, Greece)
Estradiol as a key factor affecting the synthesis of matrix proteoglycans implicated in breast cancer
13:00-14:30 • Lunch
Symposium 5: ECM Alterations in Health and Disease
Chairperson/discussion leader: K. Sugahara
• Invited Lectures (L24-L26)
14:30-15:10 A. Hjerpe (Karolinska Institute, Sweden)
Malignant Mesothelioma – a Connective Tissue Tumour with Proteoglycan-depending Differentiation Patterns
15:10-15:50 I. Pucci-Minafra (University of Palermo, Italy)
Proteomic modulation in breast cancer cells induced by microenvironment components
15:50-16:30 N. Karamanos (University of Patras, Greece)
Differential Expression and Structural Modifications of Proteoglycans and Metalloproteinases in Cancer – Molecular Targets
16:30-17:00 • Coffee break
• Selected talks (ST25-ST33)
17:00-17:10 A. Zafiropoulos (University of Crete, Greece)
MG63 osteosarcoma cells evade the decorin-induced growth arrest by the protracted expression and activation of EGFR
17:10-17:20 E. Hadler-Olsen (University of Tromsø, 9037 Tromsø, Norway)
Orthotopic and heterotopic growth of cancer cells, - a mouse model to study the influence of extracellular matrix on the expression of MMPs
17:20-17:30 R. Elenjord (University of Tromsø, Norway)
Bisphosponates down-regulate the expression of matrix metalloproteinases in osteosarcoma cell lines
17:30-17:40 I. Kanakis (University of Patras, Greece)
Correlation between inhibition of osteoclastic bone resorption by zoledronic acid and reduction of serum levels of hyaluronan in breast cancer patients with skeletal metastasis
17:40-17:50 D. Vynios (University of Patras, Greece)
Biochemical and molecular studies of extracellular macromolecular components in squamous cell laryngeal carcinoma
17:50-18:00 N. Malla (University of Tromsø, Norway)
Covalent binding of proMMP-9 to proteoglycan core protein affects the interaction with collagen I and gelatine
18:00-18:10 N. Obermajer (University of Ljubljana, Slovenia)
Immunosupressive activity of cysteine protease cathepsin x via activation of beta-2 integrins
18:10-18:20 S. Tamkovich (Institute of Chemical Biology and Fundamental Medicine, Russia)
Influence of protease activity on DNA concentration in human blood
18:20-18:30 M. Lapierre (INSERM, Paris, 75010, France)
Opposing role of the proprotein convertases Furin and PACE4 on breast cancer cells malignant phenotypes: Role of TIMP-1
19:30 • Dinner
22:00 • Transportation to Hotels
Saturday, 26 May
08:30 • Transportation from Hotels to Conference and Cultural Center
Symposium 6: Ageing / Cancer and Proteases
Chairperson/discussion leader: H. Nagase
• Invited Lectures (L27-L29)
09:00-09:40 J. P. Pujol (University of Caen, France)
Molecular Mechanisms of Cartilage Destruction and Repair
09:40-10:20 H. Nagase (Imperial College London, UK)
Elucidating the Functions of Non-Catalytic Domains of Metalloproteinases that Degrade Extracellular Matrix
10:20-11:00 S. Menashi (CNRS , Universite Paris 12, Creteil, France)
Role of the Matrix Metalloproteinases Inducer EMMPRIN in Wound Healing
• Selected talks (ST34)
11:00-11:10 N. H. Lim (Imperial College London,UK)
Investigation of the effect of ADAMTS-selective TIMP mutants on the aggrecanolytic and collagenolytic activity in human osteoarthritic cartilage
11:10-11:20 • D. Heinegård and J.P. Pujol will deliver the final keynote address to conclude FEBS-MPST2007 Course
11:20-12:00 • Farewell Party
12:00 • Transportation from Patras to Athens Airport